Use of CHROMagar Candida medium for isolation of yeasts from dental samples.
Identifieur interne : 00A456 ( Main/Exploration ); précédent : 00A455; suivant : 00A457Use of CHROMagar Candida medium for isolation of yeasts from dental samples.
Auteurs : D. Beighton ; R. Ludford ; D T Clark ; S R Brailsford ; C L Pankhurst ; G F Tinsley ; J. Fiske ; D. Lewis ; B. Daly ; N. KhalifaSource :
- Journal of Clinical Microbiology [ 0095-1137 ] ; 1995.
Descripteurs français
- KwdFr :
- MESH :
- isolement et purification : Candida, Saccharomyces cerevisiae.
- microbiologie : Bouche édentée, Candidose buccale, Caries dentaires, Maladies de la bouche.
- Humains, Milieux de culture, Prothèse dentaire complète.
English descriptors
- KwdEn :
- MESH :
- chemical : Culture Media.
- isolation & purification : Candida, Saccharomyces cerevisiae.
- microbiology : Candidiasis, Oral, Dental Caries, Mouth Diseases, Mouth, Edentulous.
- Denture, Complete, Humans.
Abstract
A new differential medium, CHROMagar Candida, for the isolation of clinically important yeasts was investigated to determine its usefulness in facilitating the study of oral yeasts. The recovery of yeasts on the medium was not significantly different from the recovery on Sabouraud dextrose agar. The identities of 450 green colonies on CHROMagar Candida, presumptively identified as Candida albicans on the basis of the manufacturer's instructions, were confirmed by testing for beta-N-acetylgalactosaminidase. Candida tropicalis also formed distinctive colonies, and other yeasts including Candida (Torulopsis) glabrata, Candida Parapsilosis, Candida Magnoliae, Candida lusitaniae, Candida Famata, Candida kefir, and Saccharomyces cerevisiae were readily distinguished from C. albicans and C. tropicalis isolates. CHROMagar Candida is a very useful medium, and its use will facilitate the study of yeasts associated with dental diseases.
Url:
PubMed: 8576366
PubMed Central: 228627
Affiliations:
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Le document en format XML
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<term>Culture Media</term>
<term>Dental Caries (microbiology)</term>
<term>Denture, Complete</term>
<term>Humans</term>
<term>Mouth Diseases (microbiology)</term>
<term>Mouth, Edentulous (microbiology)</term>
<term>Saccharomyces cerevisiae (isolation & purification)</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>Bouche édentée (microbiologie)</term>
<term>Candida (isolement et purification)</term>
<term>Candidose buccale (microbiologie)</term>
<term>Caries dentaires (microbiologie)</term>
<term>Humains</term>
<term>Maladies de la bouche (microbiologie)</term>
<term>Milieux de culture</term>
<term>Prothèse dentaire complète</term>
<term>Saccharomyces cerevisiae (isolement et purification)</term>
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<keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Candida</term>
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<term>Saccharomyces cerevisiae</term>
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<term>Candidose buccale</term>
<term>Caries dentaires</term>
<term>Maladies de la bouche</term>
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<term>Dental Caries</term>
<term>Mouth Diseases</term>
<term>Mouth, Edentulous</term>
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<term>Humans</term>
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<front><div type="abstract" xml:lang="en"><p>A new differential medium, CHROMagar Candida, for the isolation of clinically important yeasts was investigated to determine its usefulness in facilitating the study of oral yeasts. The recovery of yeasts on the medium was not significantly different from the recovery on Sabouraud dextrose agar. The identities of 450 green colonies on CHROMagar Candida, presumptively identified as Candida albicans on the basis of the manufacturer's instructions, were confirmed by testing for beta-N-acetylgalactosaminidase. Candida tropicalis also formed distinctive colonies, and other yeasts including Candida (Torulopsis) glabrata, Candida Parapsilosis, Candida Magnoliae, Candida lusitaniae, Candida Famata, Candida kefir, and Saccharomyces cerevisiae were readily distinguished from C. albicans and C. tropicalis isolates. CHROMagar Candida is a very useful medium, and its use will facilitate the study of yeasts associated with dental diseases.</p>
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<name sortKey="Clark, D T" sort="Clark, D T" uniqKey="Clark D" first="D T" last="Clark">D T Clark</name>
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<name sortKey="Fiske, J" sort="Fiske, J" uniqKey="Fiske J" first="J" last="Fiske">J. Fiske</name>
<name sortKey="Khalifa, N" sort="Khalifa, N" uniqKey="Khalifa N" first="N" last="Khalifa">N. Khalifa</name>
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